Separation Characteristics of a Phosphatidylcholine-Coated ODS Column for Direct Sample Injection Analysis of Biological Fluid Samples
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- Hasegawa Takuya
- Department of Applied Chemistry, Graduate School of Engineering, Nagoya University
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- Fukumoto Yasuharu
- Department of Applied Chemistry, Graduate School of Engineering, Nagoya University
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- Ishise Jotaro
- Department of Applied Chemistry, Graduate School of Engineering, Nagoya University
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- Hattori Ryota
- Department of Applied Chemistry, Graduate School of Engineering, Nagoya University
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- Zhu Yanbei
- Department of Applied Chemistry, Graduate School of Engineering, Nagoya University
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- Umemura Tomonari
- Department of Applied Chemistry, Graduate School of Engineering, Nagoya University
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- Haraguchi Hiroki
- Department of Applied Chemistry, Graduate School of Engineering, Nagoya University
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An octadecylsilyl silica (ODS) column coated with phosphatidylcholine (PC) was prepared by using a dynamic coating method, and its separation characteristics were evaluated for direct sample injection analysis of biological fluids. First, fundamental separation behaviors of various kinds of large and small molecules/ions were examined by HPLC using the PC-coated ODS column with UV absorption detection, for which pure water was used as a mobile phase. In the experiments, proteins (large molecules) were rapidly eluted in the range of 2.07 min (dead time)–3.20 min, while small molecules, such as inorganic anions, amino acids, organic acids, and organic drugs, were eluted after 3.50 min. The fact that proteins and small molecules/ions were separated on the PC-coated ODS column suggested that the present column can be used for direct sample injection analysis of biological fluid samples, such as urine and blood serum. Thus, the PC-coated ODS column was used in the analysis of arsenic species in urine and in the analysis of nicotinamide, theophylline, tryptophan, and caffeine in a model blood serum solution without any sample pretreatment.
収録刊行物
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- Bulletin of the Chemical Society of Japan
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Bulletin of the Chemical Society of Japan 80 (2), 329-334, 2007
公益社団法人 日本化学会
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詳細情報 詳細情報について
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- CRID
- 1390001204143172864
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- NII論文ID
- 130004152257
- 10018518107
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- NII書誌ID
- AA00580132
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- ISSN
- 13480634
- 00092673
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- NDL書誌ID
- 8640589
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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