Cultivation characteristics of denitrification by thermophilic Geobacillus sp. strain TDN01
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- Mishima Masatomo
- Shibaura Institute of Technology
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- Iwata Kenichi
- Shibaura Institute of Technology
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- Nara Kota
- Shibaura Institute of Technology
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- Matsui Toru
- Fundamental Technology Department, Technology Research Institute, Tokyo Gas Co., Ltd.
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- Shigeno Toshiya
- Tsukuba Environmental Microorganism Institute
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- Omori Toshio
- Shibaura Institute of Technology
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説明
Nine thermophilic denitrification bacteria were isolated from field soil, mud, and spa samples. The alignment of 16S rDNA showed that all were identical to the genus Geobacillus. Two of the bacteria produced N2O and N2 gas and the other seven strains produced N2 gas from nitrate. We examined the growth substrates for Geobacillus TDN01 and determined that sodium succinate, pyruvate, formate, acetate, glycerol, glucose, sucrose, and cellobiose well supported growth of the isolate. Growth occurred under the following concentration of NO3- and phosphate: 10-60 mmol/L, and 0.1-50 mmol/L, respectively. Thermophilic TDN01 grown on sodium succinate accumulated nitrite. A time course of denitrification by Geobacillus TDN01 in a jar fermentor revealed that maintaining a pH of around 7 is important for denitrification without accumulating NO2. The NO3- and NO2- consumption ratios of Geobacillus were 44-75 and 9-41 times higher, respectively, than those of Pseudomonas stutzeri JCM 5965T.
収録刊行物
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- The Journal of General and Applied Microbiology
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The Journal of General and Applied Microbiology 55 (2), 81-86, 2009
公益財団法人 応用微生物学・分子細胞生物学研究奨励会
