Sirtuin A regulates secondary metabolite production by <i>Aspergillus nidulans</i>
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- Itoh Eriko
- Faculty of Life and Environmental Sciences, University of Tsukuba
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- Shigemoto Ryosuke
- Faculty of Life and Environmental Sciences, University of Tsukuba
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- Oinuma Ken-Ichi
- Faculty of Life and Environmental Sciences, University of Tsukuba
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- Shimizu Motoyuki
- Faculty of Life and Environmental Sciences, University of Tsukuba
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- Masuo Shunsuke
- Faculty of Life and Environmental Sciences, University of Tsukuba
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- Takaya Naoki
- Faculty of Life and Environmental Sciences, University of Tsukuba
Bibliographic Information
- Other Title
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- Sirtuin A regulates secondary metabolite production by Aspergillus nidulans
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Abstract
<p>Late-stage cultures of filamentous fungi under nutrient starvation produce valuable secondary metabolites such as pharmaceuticals and pigments, as well as deleterious mycotoxins, all of which have remarkable structural diversity and wide-spectrum bioactivity. The fungal mechanisms regulating the synthesis of many of these compounds are not fully understood, but sirtuin A (SirA) is a key factor that initiates production of the secondary metabolites, sterigmatocystin and penicillin G, by Aspergillus nidulans. Sirtuin is a ubiquitous NAD+-dependent histone deacetylase that converts euchromatin to heterochromatin and silences gene expression. In this study, we have investigated the transcriptome of a sirA gene disruptant (SirAΔ), and found that SirA concomitantly repressed the expression of gene clusters for synthesizing secondary metabolites and activated that of others. Extracts of SirAΔ cultures grown on solid agar and analyzed by HPLC indicated that SirA represses the production of austinol, dehydroaustinol and sterigmatocystin. These results indicated that SirA is a transcriptional regulator of fungal secondary metabolism.</p>
Journal
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- The Journal of General and Applied Microbiology
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The Journal of General and Applied Microbiology 63 (4), 228-235, 2017
Applied Microbiology, Molecular and Cellular Biosciences Research Foundation
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Details 詳細情報について
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- CRID
- 1390001204145345152
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- NII Article ID
- 130006067742
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- NII Book ID
- AA00698664
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- ISSN
- 13498037
- 00221260
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- HANDLE
- 2241/00148410
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- PubMed
- 28674377
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- Text Lang
- en
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- Data Source
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- JaLC
- IRDB
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed