Nitrile-synthesizing enzyme: Screening, purification and characterization
-
- Kumano Takuto
- Institute of Applied Biochemistry, and Graduate School of Life and Environmental Sciences, University of Tsukuba
-
- Suzuki Takahisa
- Division of Applied Life Science, Graduate School of Agriculture, Kyoto University
-
- Shimizu Sakayu
- Division of Applied Life Science, Graduate School of Agriculture, Kyoto University
-
- Kobayashi Michihiko
- Institute of Applied Biochemistry, and Graduate School of Life and Environmental Sciences, University of Tsukuba
この論文をさがす
説明
Cyanide is known as a toxic compound for almost all living organisms. We have searched for cyanide-resistant bacteria from the soil and stock culture collection of our laboratory, and have found the existence of a lot of microorganisms grown on culture media containing 10 mM potassium cyanide. Almost all of these cyanide-resistant bacteria were found to show β-cyano-L-alanine (β-CNAla) synthetic activity. β-CNAla synthase is known to catalyze nitrile synthesis: the formation of β-CNAla from potassium cyanide and O-acetyl-L-serine or L-cysteine. We found that some microorganisms were able to detoxify cyanide using O-methyl-DL-serine, O-phospho-L-serine and β-chloro-DL-alanine. In addition, we purified β-CNAla synthase from Pseudomonas ovalis No. 111 in nine steps, and characterized the purified enzyme. This enzyme has a molecular mass of 60,000 and appears to consist of two identical subunits. The purified enzyme exhibits a maximum activity at pH 8.5–9.0 at an optimal temperature of 40–50°C. The enzyme is specific for O-acetyl-L-serine and β-chloro-DL-alanine. The Km value for O-acetyl-L-serine is 10.0 mM and Vmax value is 3.57 μmol/min/mg.
収録刊行物
-
- The Journal of General and Applied Microbiology
-
The Journal of General and Applied Microbiology 62 (4), 167-173, 2016
公益財団法人 応用微生物学・分子細胞生物学研究奨励会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390001204145494272
-
- NII論文ID
- 130006770738
-
- NII書誌ID
- AA00698664
-
- ISSN
- 13498037
- 00221260
-
- HANDLE
- 2241/00144416
-
- PubMed
- 27250663
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- IRDB
- Crossref
- PubMed
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可