Non-genetic variability of division cycle and growth of isolated individual cells in on-chip culture system
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- INOUE Ippei
- Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo
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- WAKAMOTO Yuichi
- Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo
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- YASUDA Kenji
- Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo
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To investigate the non-genetic variability of the division cycle and growth of single cells, we compared pairs of Eschertchia coli daughter cells' growth and division time under isolated conditions using a newly developed on-chip culture system. The advantages of the system are: (i) continuous cultivation of isolated single cells or a group of cells in μm-sized micro-cages under isolated contamination-free and exchangeable medium conditions, and (ii) continuous observation and comparison of those floating daughter cells at a spatial resolution of 0.2μm by phase-contrast/fluorescence microscopy and digital image processing. Using this system, cell growth and division time of daughter cells from an isolated mother cell were measured. A broad distribution of the cell division time and the division time differences of two daughter cells from the same mother cells were found. They are not attributable to a difference in DNA. The same tendency of broad distribution of the division time and the division time differences suggests the involvement of a probabilistic process to start the division.
収録刊行物
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- Proceedings of the Japan Academy. Ser. B: Physical and Biological Sciences
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Proceedings of the Japan Academy. Ser. B: Physical and Biological Sciences 77 (8), 145-150, 2001
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詳細情報 詳細情報について
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- CRID
- 1390001204145522304
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- NII論文ID
- 130000907922
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- NII書誌ID
- AA00785485
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- ISSN
- 13492896
- 03862208
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- NDL書誌ID
- 5987147
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可