Recent advances in rice genome and chromosome structure research by fluorescence in situ hybridization (FISH)
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- OHMIDO Nobuko
- Graduate School of Human Development and Environment, Kobe University
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- FUKUI Kiichi
- Department of Biotechnology, Graduate School of Engineering, Osaka University
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- KINOSHITA Toshiro
- Professor Emeritus, Hokkaido University
書誌事項
- タイトル別名
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- Recent advances in rice genome and chromosome structure research by fluorescence <i>in situ</i> hybridization (FISH)
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説明
Fluorescence in situ hybridization (FISH) is an effective method for the physical mapping of genes and repetitive DNA sequences on chromosomes. Physical mapping of unique nucleotide sequences on specific rice chromosome regions was performed using a combination of chromosome identification and highly sensitive FISH. Increases in the detection sensitivity of smaller DNA sequences and improvements in spatial resolution have ushered in a new phase in FISH technology. Thus, it is now possible to perform in situ hybridization on somatic chromosomes, pachytene chromosomes, and even on extended DNA fibers (EDFs). Pachytene-FISH allows the integration of genetic linkage maps and quantitative chromosome maps. Visualization methods using FISH can reveal the spatial organization of the centromere, heterochromatin/euchromatin, and the terminal structures of rice chromosomes. Furthermore, EDF-FISH and the DNA combing technique can resolve a spatial distance of 1 kb between adjacent DNA sequences, and the detection of even a 300-bp target is now feasible. The copy numbers of various repetitive sequences and the sizes of various DNA molecules were quantitatively measured using the molecular combing technique. This review describes the significance of these advances in molecular cytology in rice and discusses future applications in plant studies using visualization techniques.<br><br>(Communicated by Koichiro TSUNEWAKI, M.J.A.)
収録刊行物
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- Proceedings of the Japan Academy. Ser. B: Physical and Biological Sciences
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Proceedings of the Japan Academy. Ser. B: Physical and Biological Sciences 86 (2), 103-116, 2010
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詳細情報 詳細情報について
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- CRID
- 1390001204146952832
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- NII論文ID
- 130000168767
- 40016959266
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- NII書誌ID
- AA00785485
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- COI
- 1:STN:280:DC%2BC3c7gsFGhug%3D%3D
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- ISSN
- 13492896
- 03862208
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- NDL書誌ID
- 10547316
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- PubMed
- 20154468
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可