Reflections on my career in analytical chemistry and biochemistry
-
- SWEELEY Charles C.
- Department of Biochemistry, Michigan State University
Search this article
Abstract
My career has been focused in two major areas, analytical chemistry and biochemistry of complex lipids and glycoconjugates. Included here are the pioneering work on the gas chromatography of long-chain sphingolipid bases, carbohydrates, steroids and urinary organic acids. Mass spectrometry was utilized extensively in structural studies of sphingolipids, fatty acids, carbohydrates, steroids, urinary organic acids, polyisoprenoid alcohols, and juvenile hormone. Computer systems were developed for the acquisition and analysis of mass spectra, and were used for development of automated metabolic profiling of complex mixtures of metabolites. Fabry’s disease was discovered to be a glycosphingolipidosis. Enzymes of lysosomal metabolism of glycosphingolipids were purified, characterized, and used in one of the first demonstrations of the feasibility of enzyme replacement therapy in a lysosomal storage disorder (Fabry’s disease). Extracellular sialidases were studied to evaluate the hypothesis that they might be involved in the regulation of membrane growth factor receptors. The enzyme for hematoside synthesis was purified and characterized.<BR><BR>(Communicated by Kunihico SUZUKI, M.J.A.)
Journal
-
- Proceedings of the Japan Academy, Series B
-
Proceedings of the Japan Academy, Series B 86 (8), 822-836, 2010
The Japan Academy
- Tweet
Keywords
Details 詳細情報について
-
- CRID
- 1390001204147921152
-
- NII Article ID
- 130000442354
-
- NII Book ID
- AA00785485
-
- ISSN
- 13492896
- 03862208
-
- NDL BIB ID
- 10863680
-
- Text Lang
- en
-
- Data Source
-
- JaLC
- NDL
- Crossref
- CiNii Articles
-
- Abstract License Flag
- Disallowed