Nucleotide sequence of SN-07 chromophore binding site.

DOI DOI PubMed Open Access
  • KIMURA KEN-ICHI
    Research Institute of Life Science, Snow Brand Milk Products Co., Ltd.
  • MORINAGA TOMONORI
    Research Institute of Life Science, Snow Brand Milk Products Co., Ltd.
  • MIYATA NOBUO
    Research Institute of Life Science, Snow Brand Milk Products Co., Ltd.
  • KAWANISHI GOSEI
    Research Institute of Life Science, Snow Brand Milk Products Co., Ltd.

Bibliographic Information

Other Title
  • Specific binding of SN-07 chromophore, an anthracycline antibiotic, to DNA.

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Description

DNase I footprinting was used to investigate binding sites for SN-07 chromophore on DNA fragments prepared from pBR322. Six sites were protected on about 150 base pair DNA fragments by SN-07 chromophore, but not by related anthracycline antibiotics from DNase I digestion. All the protected sites contained the dinucleotide sequence 5'-GC-3', but no other regularities could be discerned. A drug-induced conformational change of DNA was suggested by enhancement of DNase I sensitivity between the protected sites. These results support covalent interaction of the carbinolamine function of SN-07 chromophore to 2-amino group of guanine residues.

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Details 詳細情報について

  • CRID
    1390001204148563328
  • NII Article ID
    110006324406
  • NII Book ID
    AA00515312
  • DOI
    10.7164/antibiotics.42.1838
    10.1271/bbb1961.53.1805
  • COI
    1:CAS:528:DyaK3cXnt1CgtA%3D%3D
    1:CAS:528:DyaL1MXlt1Kmtb8%3D
  • ISSN
    18811469
    18811280
    00218820
    00021369
  • PubMed
    2621165
  • Text Lang
    en
  • Article Type
    journal article
  • Data Source
    • JaLC
    • Crossref
    • PubMed
    • CiNii Articles
    • OpenAIRE
  • Abstract License Flag
    Disallowed

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