ChemInform Abstract: Effect of a Novel Antibiotic, Heliquinomycin, on DNA Helicase and Cell Growth.
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- CHINO MAKOTO
- Institute of Microbial Chemistry
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- NISHIKAWA KIYOHIRO
- Pharmaceuticals Group, Nippon Kayaku Co. Ltd.
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- YAMADA AYUMI
- Division of Cellular Biology, Institute for Molecular and Cellular Biology, Osaka University
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- OHSONO MICHIYO
- Institute for Chemotherapy
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- SAWA TSUTOMU
- Institute of Microbial Chemistry
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- HANAOKA FUMIO
- Division of Cellular Biology, Institute for Molecular and Cellular Biology, Osaka University
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- ISHIZUKA MASAAKI
- Institute for Chemotherapy
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- TAKEUCHI TOMIO
- Institute of Microbial Chemistry
書誌事項
- タイトル別名
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- Effect of a Novel Antibiotic, Heliquinomycin, on DNA Helicase and Cell Growth.
- 公開日
- 1998
- 資源種別
- journal article
- DOI
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- 10.7164/antibiotics.51.480
- 10.1002/chin.199843270
- 公開者
- 公益財団法人 日本感染症医薬品協会
この論文をさがす
説明
Heliquinomycin, a novel microbial product, was found to inhibit a human DNA helicase enzyme isolated from HeLa S3 cells at concentrations of 5 to 10 μg/ml. In contrast, adriamycin, etoposide and cisplatin did not inhibit this enzyme at the concentrations tested. Furthermore, the replication and repair of SV40 chromosome were not affected at heliquinomycin concentration of 50 μg/ml. The topoisomerase II and I enzymes were inhibited at 30 μg/ml and 100 μg/ml of heliquinomycin, respectively. Heliquinomycin inhibited the growth of HeLa S3, KB, LS180, K562 and HL60 human tumor cell lines at IC50 values of 0.96 to 2.8 μg/ml. In addition, the growth of adriamycin and cisplatin resistant P388 cell lines were inhibited at similar concentrations. Heliquinomycin inhibited both DNA and RNA synthesis in cell culture but did not inhibit protein synthesis. HeLa S3 cells were arrested at the G2/M phase by heliquinomycin. These studies suggest that heliquinomycin is a selective inhibitor of a cellular DNA helicase and in turn, inhibits growth of tumor cell lines.
収録刊行物
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- The Journal of Antibiotics
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The Journal of Antibiotics 51 (5), 480-486, 1998
公益財団法人 日本感染症医薬品協会