Studies on the Biosynthesis of Bialaphos. Biochemical Mechanism of C-P Bond Formation: Discovery of Phosphonopyruvate Decarboxylase which Catalyzes the Formation of Phosphonoacetaldehyde from Phosphonopyruvate.

DOI PubMed 被引用文献2件 オープンアクセス
  • NAKASHITA HIDEO
    Institute of Molecular and Cellular Biosciences, The University of Tokyo Present address: The Institute of Physical and Chemical Research (RIKEN)
  • WATANABE KEI
    Institute of Molecular and Cellular Biosciences, The University of Tokyo
  • HARA OSAMU
    Pharmaceutical Research Center, Meiji Seika Kaisha Ltd.
  • HIDAKA TOMOMI
    Institute of Molecular and Cellular Biosciences, The University of Tokyo
  • SETO HARUO
    Institute of Molecular and Cellular Biosciences, The University of Tokyo

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説明

The biosynthetic step following the phosphoenolpyruvate (PEP) phosphomutase reaction which forms a C-P bond of bialaphos was proven by the identification of phosphonopyruvate (PnPy) and phosphonoacetaldehyde (PnAA) as intermediates in the culture broth of Streptomyces hygroscopicus, a producing organism of bialaphos, and by detection of enzymatic decarboxylation of PnPy to PnAA. Purified PnPy decarboxylase turned out to require thiamine diphosphate and Mg2+ as cofactors. PnPy decarboxylase drives the unfavorable forward reaction to form PnPy catalyzed by PEP phosphomutase and is suggested to be essential to C-P compound biosynthesis.

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詳細情報 詳細情報について

  • CRID
    1390001204150308864
  • NII論文ID
    130003503271
  • DOI
    10.7164/antibiotics.50.212
  • COI
    1:CAS:528:DyaK2sXisFentb0%3D
  • ISSN
    18811469
    00218820
  • PubMed
    9127192
  • 本文言語コード
    en
  • データソース種別
    • JaLC
    • Crossref
    • CiNii Articles
    • OpenAIRE
  • 抄録ライセンスフラグ
    使用不可

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