Induction of single strand scission in bacteriophage .PHI.X174 replicative form I DNA by mitomycin C.
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- UEDA KAZUMITSU
- Laboratory of Biochemistry, Department of Agricultural Chemistry, Kyoto University
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- MORITA JUNJI
- Laboratory of Biochemistry, Department of Agricultural Chemistry, Kyoto University
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- KOMANO TOHRU
- Laboratory of Biochemistry, Department of Agricultural Chemistry, Kyoto University
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説明
The action of mitomycin C on double-stranded replicative form I DNA (RF I DNA; supercoiled, covalently closed, circular duplex DNA) of bacteriophage φX174 was investigated using the technique of agarose gel electrophoresis. Mitomycin C reduced with sodium hydrosulfite (sodium dithionite, Na2S2O4) caused single strand scission in φX174 RF I DNA in the presence of Cu2+. Cu2+ was essential for this DNA cleavage action, and other transition metal ions such as Fe2+, Fe3+, Mn2+, Co2+ and Zn2+ were of no effect. This DNA strand scission was inhibited by catalase (EC 1.11.1.6) and various radical scavengers. This DNA strand scission was caused by free oxygen radicals generated during autoxidation of reduced mitomycin C in the presence of Cu2+.
収録刊行物
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- The Journal of Antibiotics
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The Journal of Antibiotics 34 (3), 317-322, 1981
公益財団法人 日本感染症医薬品協会