MODE OF ACTION OF XANTHOCILLIN X MONOMETHYLETHER ON MULTIPLICATION OF NEWCASTLE DISEASE VIRUS IN CULTURED CELLS

DOI PubMed オープンアクセス
  • TAKATSUKI AKIRA
    Laboratory of Microbiology, Department of Agricultural Chemistry, the University of Tokyo
  • TAMURA GAKUZO
    Laboratory of Microbiology, Department of Agricultural Chemistry, the University of Tokyo
  • ARIMA KEI
    Laboratory of Microbiology, Department of Agricultural Chemistry, the University of Tokyo

書誌事項

タイトル別名
  • STUDIES ON ANTIVIRAL AND ANTITUMOR ANTIBIOTICS. XV

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説明

The mechanism of the action of xanthocillin X monomethylether (XME) on multiplication of Newcastle disease virus (NDV) in cultured cells was studied. The inhibitory effect of XME was exerted whenever the antibiotic (15μg/ml) was added during the viral one-step growth cycle, and the effect could be reversed after removal of the antibiotic by washing. A lag period of approximately 5 hours was observed before the restoration of hemagglutinin synthesis had occurred. Pretreatment of host cells with XME for 7 hours and subsequent removal of the antibiotic did not affect velocity and yield of virus production. XME completely inhibited protein synthesis in both virus infected and mock-infected cells, however total actinomycin D-insensitive RNA synthesis was found to be practically the same in antibiotic-treated cells as in the control. Actinomycin D-insensitive RNA synthesized in the presence of XME was more heterogeneous than the control using methylated albumin-kieselguhr (MAK) column chromatography. Antiviral activity of XME was compared with that of other antibiotics known to be inhibitors of protein synthesis. XME was found to be one of the best inhibitors among all compounds tested, due to its low cytotoxicity and low effective concentration in agar-diffusion plaque-inhibition tests.

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詳細情報 詳細情報について

  • CRID
    1390001204152868608
  • NII論文ID
    130003498171
  • DOI
    10.7164/antibiotics.22.151
  • COI
    1:CAS:528:DyaF1MXktFSlsb8%3D
  • ISSN
    18811469
    00218820
  • PubMed
    5816502
  • 本文言語コード
    en
  • 資料種別
    journal article
  • データソース種別
    • JaLC
    • Crossref
    • PubMed
    • CiNii Articles
    • OpenAIRE
  • 抄録ライセンスフラグ
    使用不可

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