Isotope Dilution Analysis for Urinary Fentanyl and Its Main Metabolite, Norfentanyl, in Patients by Isotopic Fractionation Using Capillary Gas Chromatography.

SERA Shoji, GOROMARU Tsuyoshi, SAMESHIMA Teruko, KAWASAKI Koichi, ODA Toshiyuki

doi:10.3769/radioisotopes.47.537

Isotope dilution analysis was applied to determine urinary excretion of fentanyl (FT) and its main metabolite, norfentanyl (Nor-FT), by isotopic fractionation using a capillary gas chromatograph equipped with a surface ionization detector (SID) . Urinary FT was determined quantitatively in the range of 0.4-40 ng/ml using deuterium labeled FT (FT-²H₁₉), as an internal standard.<BR>We also performed isotope dilution analysis of Nor-FT in urine. NV Alkylation was necessary to sensitively detect Nor-FT with SID. Methyl derivative was selected from 3 kinds of NV alkyl derivatives to increase sensitivity and peak resolution, and to prevent interference with urinary compound. Nor-FT concentration was quantitatively determined in the range of 10-400 ng/ml using deuterium labeled Nor-FT (Nor-FT-²H₁₀) . No endogenous compounds or concomitant drugs interfered with the detection of FT and Nor-FT in the urine of patients. The present method will be useful for pharmacokinetic studies and the evaluation of drug interactions in FT metabolism.

Isotope Dilution Analysis for Urinary Fentanyl and Its Main Metabolite, Norfentanyl, in Patients by Isotopic Fractionation Using Capillary Gas Chromatography.

Bibliographic Information

Search this article

Abstract

Journal

Citations (1)*help

References(23)*help

Keywords

Details 詳細情報について

Export

Report a problem