簡単迅速な不飽和血清鉄結合能 (UIBC) の測定法

DOI Web Site Web Site
  • 斎藤 宏
    名古屋大学医学部アイソトープ研究室
  • 三島 厚
    名古屋大学附属病院アイソトープ検査室

書誌事項

タイトル別名
  • A Rapid Method for the Determination of Unsaturated Iron-Binding Capacity (UIBC) of the Serum
  • カンタン ジンソク ナ フホウワ ケッセイテツ ケツゴウノウ UIBC ノ ソクテイホウ

この論文をさがす

抄録

UIBC was determined rapidly by using a new simple device consists of an outer test tube, which is also used for counting, and an inner tube having a rubber piston ring, which has a sponge or cotton filter inside the ring.<BR>The serum and ferric ammonium citrate 59FeFe solution are added and incubated for 15 minutes in the outer tube.<BR>Amberlite IRP-67 ion exchange resin powder is added to the iron serum solution mixture, and kept for one minute. Thereafter the inner tube is inserted and pushed down. Then the serum solution appears above the filter and the resin remains under the piston ring. The serum solution is removed and the resin is washed with water three times in a minute by pushing and pulling the inner tube. The washed resin in the tube is counted in a well-type scintillation counter. In reverse, if the resin is added onto the piston ring and the inner tube is removed, the serum solution for counting is obtained.<BR>The use of this device enables the rapid determination of UIBC without the procedures as pipetting, centrifugation, and mixing.

収録刊行物

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ