HPLC Determination of (+)-Pseudoephedrine and (-)-Ephedrine in Japanese Herbal Medicines Containing Ephedra Herb Using Solid-Phase Extraction
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- Ichikawa Makoto
- Healthcare Research Institute, Wakunaga Pharmaceutical Co., Ltd.
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- Udayama Manabu
- Healthcare Research Institute, Wakunaga Pharmaceutical Co., Ltd.
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- Imamura Kazuhiko
- Healthcare Research Institute, Wakunaga Pharmaceutical Co., Ltd.
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- Shiraishi Sumihiro
- Healthcare Research Institute, Wakunaga Pharmaceutical Co., Ltd.
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- Matsuura Hiromichi
- Healthcare Research Institute, Wakunaga Pharmaceutical Co., Ltd.
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Abstract
We developed a rapid and simple HPLC method combined with solid-phase extraction (SPE) for quantitative analysis of (+)-pseudoephedrine (PEP) and (−)-ephedrine (EP) in Japanese herbal (Kampo) medicines such as Kakkon-to, Sho-seiryu-to, Goshaku-san and Bofu-tsusho-san. SPE was performed on TOYOPAK IC-SP M containing propylsulfonic groups. Determination of PEP and EP was carried out using ion-pair reversed-phase HPLC with sodium dodecyl sulfate. N-Benzyldiethylamine was used as an internal standard. The analytical procedure was validated with regard to specificity, linearity, accuracy, and precision. These data suggest that the analytical method developed in this study is useful for quantitative analysis of PEP and EP in various formulations of Kampo medicine containing Ephedra herb.
Journal
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- Chemical and Pharmaceutical Bulletin
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Chemical and Pharmaceutical Bulletin 51 (6), 635-639, 2003
The Pharmaceutical Society of Japan
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Keywords
Details 詳細情報について
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- CRID
- 1390001204163413376
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- NII Article ID
- 110003615367
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- NII Book ID
- AA00602100
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- COI
- 1:CAS:528:DC%2BD3sXls1Kntr0%3D
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- ISSN
- 13475223
- 00092363
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- NDL BIB ID
- 6541458
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- PubMed
- 12808238
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed