Quantitative Estimation of Interaction between Carbohydrates and Concanavalin A by Surface Plasmon Resonance Biosensor.

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  • Goto Satoru
    Faculty of Pharmaceutical Sciences, University of Tokushima
  • Masuda Kyoko
    Faculty of Pharmaceutical Sciences, University of Tokushima
  • Miura Miyuki
    Faculty of Pharmaceutical Sciences, University of Tokushima
  • Kanazawa Keiko
    Faculty of Pharmaceutical Sciences, University of Tokushima
  • Sasaki Miki
    Faculty of Pharmaceutical Sciences, University of Tokushima
  • Masui Miyuki
    Faculty of Pharmaceutical Sciences, University of Tokushima
  • Shiramizu Maki
    Faculty of Pharmaceutical Sciences, University of Tokushima
  • Terada Hiroshi
    Faculty of Pharmaceutical Sciences, University of Tokushima
  • Chuman Hiroshi
    Faculty of Pharmaceutical Sciences, University of Tokushima

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Abstract

We measured the affinity of more than 20 sugars with concanavalin A (ConA) by an optical biosensor (surface plasmon resonance sensor) using asialofetuin (ASF) as an immobilized binding partner of ConA. We determined kinetic parameters of the effects of sugars on the dissociation of ConA from ASF quantitatively, and the structural requirements of the functional groups of sugars for binding with ConA. We found that the affinity of ConA for sugars is dependent on its conformation induced by interaction with the binding partner. In addition, the results showed that optical biosensor system is well mimics the interaction of ConA with sugars in biomembrane.

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