An Experimental Approach to Study the Binding Properties of Vitamin E(α-Tocopherol) during Hairless Mouse Skin Permeation
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- LEE Ae-Ri Cho
- College of Pharmacy, Duksung Women’s University
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- TOJO Kakuji
- Faculty of Computer Science and Systems Engineering, Kyushu Institute of Technology
書誌事項
- タイトル別名
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- An Experimental Approach to Study the Binding Properties of Vitamin E (.ALPHA.-Tocopherol) during Hairless Mouse Skin Permeation.
- Experimental Approach to Study the Binding Properties of Vitamin E アルファ Tocopherol during Hairless Mouse Skin Permeation
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抄録
An experimental approach to study the binding properties of vitamin E has been developed. Total vitamin E solubility in the skin was determined by a partition study, followed by in vitro skin permeation studies with whole skin and stripped skin. The amount of freely diffusable vitamin E in the diffusion process was determined from the permeation profiles of whole skin and stripped skin by employing a bi-layer model. The concentrations of vitamin E in the stratum corneum and viable dermis were determined separately. By subtracting this amount from the total concentration of vitamin E in the skin, as determined by the solubility study, the amount of bound vitamin E was determined. After skin permeation reached a steady state, the donor solution was removed and the permeation study continued (desorption study). During the entire period of the desorption experiment, the amount of vitamin E in the receptor solution hardly increased and remained constant. After the desorption experiment, vitamin E still remaining in the skin was determined by extracting with tissue solubilizer, SOLABLE, and is considered as the amount of vitamin E strongly bound in the skin. The concentrations of bound vitamin E determined by permeation and desorption studies coincided relatively well. To further investigate skin binding of vitamin E, a differential scanning calorimetry study was performed. Vitamin E-treated stratum corneum showed phase transitions at 76 and 85 °C, associated with lipid transitions. The thermal transitions associated with the lipid transition suggested interactions of vitamin E with lipid components of the skin. During skin permeation, vitamin E forms a very strong reservoir in the skin tissue and this amount of vitamin E, about 30%, exists as a bound-form with the lipid components of the stratum corneum.
収録刊行物
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- CHEMICAL & PHARMACEUTICAL BULLETIN
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CHEMICAL & PHARMACEUTICAL BULLETIN 49 (6), 659-663, 2001
公益社団法人 日本薬学会
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詳細情報 詳細情報について
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- CRID
- 1390001204163972480
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- NII論文ID
- 110003615965
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- NII書誌ID
- AA00602100
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- COI
- 1:CAS:528:DC%2BD3MXktFygsro%3D
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- ISSN
- 13475223
- 00092363
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- NDL書誌ID
- 5792646
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- PubMed
- 11411512
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可