Single Strand Conformation Polymorphism Analysis of Ras Oncogene by Capillary Electrophoresis with Laser-Induced Fluorescence Detector.
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Single strand conformation polymorphism (SSCP) analysis of the N-ras oncogene was achieved by capollary electrophoresis with a laser-induced fluorescence detector (CE-LIF) using methylcellulose as a molecular sieving agent. The PCR-amplified N-ras oncogene, which is known to have a point mutation at codon 61 in the neuroblastome, was investigated by CE-LIF combined with SSCP (SSCP-CE-LIF). A mixture of wild- and mutant-type single strand DNA fragments (103 bp) of the N-ras oncogene was separated by buffer solution containing 1.0% methylcellulose and 0.2μm fluorescent dye (YO-PRO-1) at 25°C.The SSCP-CE-LIF technique gave good resolution for wild- and mutant-type single strand DNA fragments with separation completed within 7 min. SSCP analysis using a CE system with a LIF detector was successfully applied to the detection of the one point mutation on the N-ras oncogene.
収録刊行物
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- CHEMICAL & PHARMACEUTICAL BULLETIN
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CHEMICAL & PHARMACEUTICAL BULLETIN 48 (6), 774-778, 2000
公益社団法人 日本薬学会
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詳細情報 詳細情報について
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- CRID
- 1390001204164398720
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- NII論文ID
- 130003948111
- 110003635549
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- NII書誌ID
- AA00602100
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- ISSN
- 13475223
- 00092363
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- NDL書誌ID
- 5355100
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- PubMed
- 10866135
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可