Purification of 2,3-oxidosqualene: cycloartenol cyclase from seedlings.

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  • Abe Ikuro
    Faculty of Pharmaceutical Sciences, the University of Tokyo
  • Ebizuka Yutaka
    Faculty of Pharmaceutical Sciences, the University of Tokyo
  • Sankawa Ushio
    Faculty of Pharmaceutical Sciences, the University of Tokyo

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Description

2, 3-Oxidosqualene: cycloartenol cyclase (E.C.5.4.99.8) is a membrane bound enzyme prevalent in the plant kingdom.It acts as a key enzyme in phytosterol biosynthesis.Cycloartenol cyclase was purified from pea seedlings in six steps as a soluble and homogeneous enzyme. The purified enzyme showed a single band in sodium dodecylsulphate polyacrylamide gel electrophoresis (SDS-PAGE) with a molecular weight of 55 K Da and had a Km value of 25 μM.Cycloartenol cyclase requires Triton X-100 or deoxycholate for its highest activity.

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