Optimization of an HPLC Method for Determination of Gabapentin in Dosage Forms through Derivatization with 1-Fluoro-2,4-dinitrobenzene

DOI Web Site Web Site 参考文献38件
  • Souri Effat
    Department of Medicinal Chemistry, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Medical Sciences/University of Tehran
  • Jalalizadeh Hassan
    Department of Medicinal Chemistry, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Medical Sciences/University of Tehran
  • Shafiee Abbas
    Department of Medicinal Chemistry, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Medical Sciences/University of Tehran

この論文をさがす

抄録

A rapid, sensitive and accurate high performance liquid chromatography with UV detection method was developed and validated for the quantification of gabapentin in dosage forms. Gabapentin was quantified after pre-column derivatization with 1-fluoro-2,4-dinitrobenzene. Amlodipine was used as an internal standard. The chromatographic separation was carried out on a Nova-Pak C18 column using a mixture of acetonitrile–sodium dihydrogenphosphate (pH 2.5; 0.05 M) (70 : 30, v/v) as mobile phase with UV detection at 360 nm. The method was linear over the range of 10—500 µg/ml of gabapentin (r2>0.999). The within-day and between-day precision values were in the range of 0.86—1.11%. The method was successfully used for quantitative determination and dissolution rate study of Neurontin capsules.

収録刊行物

参考文献 (38)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ