Improvements in transfection efficiency with chitosan modified PLGA nanospheres prepared by the emulsion solvent diffusion method, for gene delivery

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  • Tahara Kohei
    Laboratory of Pharmaceutical Engineering, School of Pharmacy, Aichi Gakuin University Graduate School of Pharmaceutical Sciences, Nagoya City University
  • Sakai Takeshi
    Laboratory of Pharmaceutical Engineering, Gifu Pharmaceutical University
  • Yamamoto Hiromitsu
    Laboratory of Pharmaceutical Engineering, School of Pharmacy, Aichi Gakuin University
  • Takeuchi Hirofumi
    Laboratory of Pharmaceutical Engineering, Gifu Pharmaceutical University
  • Hirashima Naohide
    Graduate School of Pharmaceutical Sciences, Nagoya City University
  • Kawashima Yoshiaki
    Laboratory of Pharmaceutical Engineering, School of Pharmacy, Aichi Gakuin University

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  • Improvements in Transfection Efficiency with Chitosan Modified Poly(DL-lactide-co-glycolide) Nanospheres Prepared by the Emulsion Solvent Diffusion Method, for Gene Delivery

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説明

This study sought to evaluate the in vitro transfection efficiency of plasmid DNA (pDNA)-loaded chitosan-modified poly(DL-lactide-co-glycolide) nanospheres (CS-PLGA NS) in a gene-delivery system. Using the emulsion solvent diffusion (ESD) method, pDNA-loaded PLGA NS was prepared and the surface of the PLGA NS was modified by binding to CS. Gene transfection ability of CS-PLGA NS was examined in A549 cells. The luciferase gene was used as a reporter gene. The pattern of luciferase activity by pDNA-loaded CS-PLGA NS was initially weak, but gradually grew stronger before decreasing activity. These phenomena should be in accordance with the sustained-release profile of pDNA from PLGA NS in the cytosol and the pDNA protection against DNase. Positively charged CS-PLGA NS was found, by 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt (MTS) assay, not to exhibit cytotoxicity on A549 cells. These results suggest that CS-PLGA NS are potential contributors to efficient pDNA delivery due to their increased interactions with cells and lack of cytotoxic effects.

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