Traditional Processing Strongly Affects Metabolite Composition by Hydrolysis in Rehmannia glutinosa Roots

DOI Web Site Web Site 被引用文献2件 参考文献22件
  • Chang Wen-Te
    School of Chinese Medicine Resources, China Medical University
  • Choi Young Hae
    Division of Pharmacognosy, Section Metabolomics, Institute of Biology, Leiden University
  • Van der Heijden Rob
    Division of Analytical Biosciences, Leiden/Amsterdam Center for Drug Research, Centre for Medical Systems Biology
  • Lee Meng-Shiou
    School of Chinese Medicine Resources, China Medical University
  • Lin Ming-Kuem
    School of Chinese Medicine Resources, China Medical University
  • Kong Hongwei
    Division of Analytical Biosciences, Leiden/Amsterdam Center for Drug Research, Centre for Medical Systems Biology National Chromatographic R&A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences
  • Kim Hye Kyong
    Division of Pharmacognosy, Section Metabolomics, Institute of Biology, Leiden University
  • Verpoorte Robert
    Division of Pharmacognosy, Section Metabolomics, Institute of Biology, Leiden University
  • Hankemeier Thomas
    Division of Analytical Biosciences, Leiden/Amsterdam Center for Drug Research, Centre for Medical Systems Biology
  • Van der Greef Jan
    Division of Analytical Biosciences, Leiden/Amsterdam Center for Drug Research, Centre for Medical Systems Biology SU BioMedicine
  • Wang Mei
    SU BioMedicine Department of Applied Plant Science, TNO Quality of Life

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The processing of biological raw materials is considered to have an important role in the therapeutic application in Traditional Chinese Medicine. The root of Rehmannia glutinosa has to be processed by nine cycles of rice wine immersing, steaming and drying before using in clinical applications. In order to understand the chemical changes resulting from the processing, a comprehensive analysis of Rehmanniae radix was made using 1H-NMR and Fourier transform (FT)-mass spectrometry in combination with multivariate data analysis. After 1H-NMR and principle component analysis, hydrolysis was found to be the major chemical process during the treatments. Catalpol, raffinose and stachyose levels gradually decreased during processing, whereas monosaccharides including galactose and glucose were found to be higher in processed roots. The metabolic profile changed gradually through the processing cycles although the differences became smaller after the fifth processing cycle. The positive and negative ion-mode mass spectra by high resolution FT-MS revealed several series of ion clusters with mass differences of 162.053 Da, indicating a difference of a hexose moiety. During the processing, the number and signal intensity of the smaller glycosides were increased. Therefore, these results indicate that the fresh Rehmanniae radix is rich in polysaccharides, which are hydrolyzed during the processing.

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