The detection of feline herpesvirus-1 (FHV-1) using polymerase chain reaction (PCR).

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  • Ｐｏｌｙｍｅｒａｓｅ　ｃｈａｉｎ　ｒｅａｃｔｉｏｎ（ＰＣＲ）によるネコのヘルペスウイルス１型（ＦＨＶ‐１）の検出について

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We amplified the tymidine kinase region of feline herpesvirus-1 (FHV-1) genomes of several strains by the polymerase chain reaction (PCR) using the extract of DNA with instaGene-DNA purification matrix and analyzed the PCR products by using restriction endonucleases. The detection limit of purified FHV-1 DNA using PCR was 0.5pg. The lysate of infected cells (100cells/μl) by instaGene was diluted with sterile distilled water, and this diluted lysate was analysed using PCR. DNA was detected to 100 times dilution. From these results, it may be suggested that the detected DNA is obtained from only one infected cell. DNA detection limit indicated by viral titer from the viral fluid using instaGene was 10TCID50/0.1ml. Restriction patterns of PCR product cleaved with EcoR V, Mbo II, Pvu II, Mva I and Hap II revealed same genomic variation among tested FHV-1 strains.

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