Studies on serum cholinesterase isoenzymes in laboratory animals: Serum cholinesterase isoenzyme patterns and cholinesterase activities animal species.
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- Akahori Fumiaki
- Department of Veterinary Pharmacology, School of Verterinary Medicine, Azabu University
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- Sakaguchi Kazuko
- Department of Public Health Administration, College of Enviromental Health, Azabu University
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- Kohzaki Ryuko
- Department of Public Health Administration, College of Enviromental Health, Azabu University
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- Masaoka Toshio
- Department of Veterinary Pharmacology, School of Verterinary Medicine, Azabu University
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- Arai Shigeyuki
- Department of Veterinary Pharmacology, School of Verterinary Medicine, Azabu University
Bibliographic Information
- Other Title
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- 実験動物における血清コリンエステラーゼアイソザイムの研究
- Serum cholinesterase isoenzyme patterns and cholinesterase activities animal species
Abstract
We analyzed cholinesterase (ChE) isoenzyme patterns of various laboratory animal species using electrophoresis to explore possible species-related variations and also to determine if there is a relationship between the ChE activity and the ChE isoenzyme pattern.<br>The laboratory animals that we used were Standard Wistar and Spraque-Dawley rats, Djungarian hamsters, Hartley guinea pigs, Japanese white rabbits (JW-NIBS strain), Beagle dogs, Japanese monkeys, Yorkshire pigs, Thoroughbred horses and Japanese quail. The ChE isoenzyme patterns were determined using polyacrylamide gradient gel electrophoresis (PAG-EP). The activity of serum ChE was measured using Ellman's method. The ChE isoenzymes of Wistar and Sprague-Dawley rats consisted of six separate fractions and sex- and/or age-related differences in the number of fractions were not observed. However, a sex-related variation was observed in the proportions of individual ChE isoenzyme bands for both rat strains. Also a sex-related difference was demonstrated in the serum ChE activity. The number of fractions of the ChE isoenzyme exhibited a species-related variations in the other animals. Hamsters, guinea pigs, rabbits, dogs, monkeys, pigs, horses and quails had 4, 3, 4, 3-5, 3, 3, 4 and 3 ChE isoenzyme bands, respectively. However, with the exception of the rats, the hamsters, guinea pigs, rabbits, dogs, monkeys, pigs, horses and quail did not show any sex-related difference in the proportions of their ChE isoenzyme bands or in the ChE activities.<br>Therefore, it appears that there is a relationship between the serum ChE activity and the proportion of ChE isoenzyme bands. These results suggest a possible species-related variation in the mode of polymerization of serum ChE subunits that constitute the ChE isoenzymes.
Journal
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- SEIBUTSU BUTSURI KAGAKU
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SEIBUTSU BUTSURI KAGAKU 31 (4), 233-240, 1987
Japanese Electrophoresis Society
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Keywords
Details 詳細情報について
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- CRID
- 1390001204202154624
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- NII Article ID
- 130003606790
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- ISSN
- 13499785
- 00319082
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- Text Lang
- en
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- Data Source
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- JaLC
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed