Electrophoretic analysis of alkaline phosphatase isozymes: A study on serum alkaline phosphatase in transient hyperphosphatasemia.

DOI
  • Saheki Shuichi
    Department of Clinical Laboratory Medicine, University of Ehime
  • Ochi Masaaki
    Department of Clinical Laboratory Medicine, University of Ehime
  • Takeuchi Nozomu
    Department of Clinical Laboratory Medicine, University of Ehime

Bibliographic Information

Other Title
  • ＡＬＰアイソザイム分析の現状と今後の展望　電気泳動法によるアルカリ性ホスファターゼアイソザイムの解析　一過性高アルカリ性ホスファターゼ血症における肝・骨型アルカリ性ホスファターゼのヘテロジェニティー
  • A study on serum alkaline phosphatase in transient hyperphosphatasemia
  • 一過性高アルカリ性ホスファターゼ血症における肝・骨型アルカリ性ホスファターゼのヘテロジェニティー

Description

Thin layer polyacrylamide gel electrophoresis was developed for analyzing alkaline phosphatase (ALP) isozymes by introducing 25mM N-methylglucamine as a medium, and demonstrated to have good resolution. The method enabled unequivocal characterization of ALP isozymes in combination with pre-treatment of specimens by heat treatment, sialidase digestion and WGA binding. In transient hyperphosphatasemia, ALP activities of both bone and novel liver-like types were markedly increased. The latter type of ALP was different from typical liver type in having a more anodic electrophoretic mobility and stronger affinity for WGA.

Journal

Keywords

Details 詳細情報について

  • CRID
    1390001204202413184
  • NII Article ID
    130003607004
  • DOI
    10.2198/sbk.38.143
  • ISSN
    13499785
    00319082
  • Text Lang
    ja
  • Data Source
    • JaLC
    • Crossref
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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