Studies on the Lytic Action of Microsome Fraction (Adrenalin Sensitive Lipase) of Pig Pancreas Cell on Several Eumycetes, Actionomycetes and Mycobacterium (I)

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  • Mishima Seiya
    Department of Parasitology, School of Medicine, Gifu University.

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  • 豚膵臓細胞microsome分画中のadrenalin sensitive lipaseの各種真菌, 放線菌及び細菌に対するlytic actionについて (1)

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Morisita et al (1963) found an enzyme, in the body fluid of Ascaris lumbricoides, which dissolved Trichophyton and Aspergillus. Furuhashi (1964) reported a lytic action of the body fluid on Penicillium, Streptomyces and Mycobacterium. Further investigations with Morisita et al (1965) indicated the fungi-lytic action of the body fluid had depended on lipoprotein lipase. <BR>On the other hand, no lipoprotein lipase had ever been reported in invertebrates, up to the present. Yamada (1965) isolated an enzyme from the pancreas extracts of pig, dog and rabbit, which showed fungi-lytic action and identified this enzyme as the adrenalin sensitive lipase of microsome fraction of pancreas cells. This is probably the first report on the identification of adrenalin sensitive lipase in pancreas cells. Because, no fungi-lytic action was shown in lipase isolated from pancreas. <BR>In the author's investigations, lytic activities were observed in the F-fraction containing adrenalin sensitive lipase extracted from pig pancreas, on the all of Penicillium, Aspergillus, Trichophyton, Streptomyces and Mycobacterium. Period, however, requested for the lysis of cells after the contact of enzymic solution with cell walls, differed from 15 minutes to 6 hours, respectively, in individual fungal or bacterial cells. Optical-microscopic and electron-microscopic observations on the morphological changes in cells, according to the lytic action of adrenalin sensitive lipase, were as follows; changes in the sensitivity of hyphae in staining, ghost phenomenon, swelling and rupture of cell wall, and finally the flow out of cell protoplasms.

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