Detection of Tetracyclines by Using a Thin Layer Electrophoretic Method

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  • 薄層平板を用いる高圧電気泳動法によるテトラサイクリン系抗生物質の検出法の検討
  • ハクソウ ヘイバン オ モチイル コウアツ デンキ エイドウホウ ニ ヨル テ

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An electrophoretic procedure using thin layer plates of silica-gel 60F254 (Merck) and cellulose gel (Asahi Kasei Co., Ltd., Japan) has been developed for the separation and detection of 7 tetracyclines (TC, CTC, OTC, MTC, DMCTC, DOXY and MINO). Seventeen electrolyte solutions with different pH values were examined. The results obtained were as follows.<br>1. With the silica-gel plate (10×20cm), fairly good separations of tetracyclines were obtained in 0.2M sodium phosphate (dibasic)-0.1M citric acid (20:980), pH=2.2, for 40min at 1, 000V and in 1.0M sodium carbonate-1.0M sodium bicarbonate-water (50:50:900), pH=9.8, for 60min at 500V<br>2. With the cellulose plate (10×20cm), good results were obtained when the electrophoresis was done in pyridine-acetic acid-water (1:11:89), pH=3.6, for 20min at 2, 000V and in 0.05M succinic acid-0.05M sodium tetraborate (778:222), pH=4.2, for 40min at 2, 000V.<br>3. Five tetracyclines (TC, CTC, OTC, DMCTC, MINO) except for MTC and DOXY were separated from each other under the above 4 electrophoretic conditions.<br>4. All tetracyclines on TLC plates were well detected by UV absorption measurement at 365nm.

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