Sample Preprocessing Method for Residual Quinolones in Honey Using Immunoaffinity Resin
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- IHARA Yoshiharu
- Frontier Institute Co., Ltd.
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- KATO Mihoko
- Frontier Institute Co., Ltd.
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- KODAIRA Tsukasa
- Frontier Institute Co., Ltd.
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- ITOH Shinji
- Kato Brothers Honey Co., Ltd.
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- TERAKAWA Mika
- Kato Brothers Honey Co., Ltd.
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- HORIE Masakazu
- Saitama Institute of Public Health
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- SAITO Koichi
- Department of Analytical Chemistry, Hoshi University
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- NAKAZAWA Hiroyuki
- Department of Analytical Chemistry, Hoshi University
Bibliographic Information
- Other Title
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- イムノアフィニティー担体を用いたハチミツ中の残留キノロン剤前処理法
- イムノアフィニティー タンタイ オ モチイタ ハチミツ チュウ ノ ザンリュウ キノロンザイ マエ ショリ ホウ
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Abstract
A sample preparation method was developed for determination of quinolones in honey using immunoaffinity resin. For this purpose, an immunoaffinity resin for quinolones was prepared by coupling a quinolone-specific monoclonal antibody to agarose resin. Honey samples diluted with phosphate buffer were reacted with immunoaffinity resin. After the resin was washed, quinolones were eluted with glycine-HCl. Quinolones in the eluate were determined by HPLC with fluorescence detection. No interfering peak was found on the chromatograms of honey samples. The recoveries of quinolones from samples were over 70% at fortification levels of 20 ng/g (for norfloxacin, ciprofloxacin and enrofloxacin) and 10 ng/g (for danofloxacin). The quantification limits of quinolones were 2 ng/g. This sample preprocessing method using immunoaffinity resin was found to be effective and suitable for determining residual quinolones in honey.
Journal
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- Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi)
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Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi) 50 (3), 135-139, 2009
Japanese Society for Food Hygiene and Safety
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Details 詳細情報について
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- CRID
- 1390001204223206528
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- NII Article ID
- 130000135380
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- NII Book ID
- AN00117741
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- COI
- 1:CAS:528:DC%2BD1MXotFGhsro%3D
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- ISSN
- 18821006
- 00156426
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- NDL BIB ID
- 10363467
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- Text Lang
- ja
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- Data Source
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- JaLC
- IRDB
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed