Behavior of Trp-P-1 and Its Metabolites in Rat Excreta.
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- EGOSHI Kazuo
- Kurume Shin-Ai Women's College
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- NAKAOKA Hiroshi
- Higashichikushi Junior College
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- OKA Terumi
- Kurume Shin-Ai Women's College
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- ABO Kouji
- Kurume Shin-Ai Women's College
Bibliographic Information
- Other Title
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- ラット排せつ物中でのTrp‐P‐1及びその代謝物の挙動
- ラット排泄物中でのTrp-P-1及びその代謝物の挙動
- ラット ハイセツブツ チュウ デ ノ Trp P 1 オヨビ ソノ タイシャブツ ノ キョドウ
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Abstract
To study of the behavior of Trp-P-1 and its metabolites in rat feces and urine, rats were orally administered with Trp-P-1 (750, 1,500 and 2,500 μg/rat), and excreted Trp-P-1 was analyzed using HPLC assay and bacterial mutagenicity assay. The extraction of Trp-P-1 from urine was performed by using the chloroform extraction method, and blue rayon was used for the extraction from feces.<br>When Trp-P-1 was added to rat feces and urine, the recoveries of Trp-P-1 were 85.9±3.9% and 91.3±3.7%, respectively. The extracts of feces and urine from rats administered with Trp-P-1 were individually fractionated by thin layer chromatography on C18 gel. The major mutagenic zone corresponding to Trp-P-1 was found at Rf 0.09 in both extracts, while the feces extract gave two additional mutagenic zones at Rf 0.15 and 0.20. More than 97% of the fecal mutagenic activity was due to unchanged Trp-P-1.<br>In rats administered with 750 μg of Trp-P-1, the amount of extracted Trp-P-1 and the number of His+ colonies induced by whole excreta were 81.6±7.1 μg (n=6) and (432±77)×104 for feces, and 28.7±4.9 μg and (171±28)×104 for urine. The recoveries of Trp-P-1 in the feces and urine were 10.8±0.9% and 3.8±0.7% by HPLC analysis, and 11.1±2.0% and 4.4±0.7% by mutagenicity assay respectively. The results of the two assays seemed to show similar patterns of recovery.
Journal
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- Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi)
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Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi) 42 (4), 220-225, 2001
Japanese Society for Food Hygiene and Safety
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Details 詳細情報について
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- CRID
- 1390001204223598720
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- NII Article ID
- 10009306271
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- NII Book ID
- AN00117741
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- COI
- 1:CAS:528:DC%2BD3MXnt1Wnu78%3D
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- ISSN
- 18821006
- 00156426
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- NDL BIB ID
- 5894670
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- PubMed
- 11817135
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- Text Lang
- ja
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- Data Source
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- JaLC
- IRDB
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed