HPLCによる食品中のネオテーム，アリテームおよびアスパルテームの同時分析法

DOI 日本農学文献記事索引 Web Site 被引用文献1件 参考文献2件

書誌事項

タイトル別名
  • Simultaneous Determination of Neotame, Alitame and Aspartame in Foods by HPLC
  • HPLC ニ ヨル ショクヒンチュウ ノ ネオテーム アリテーム オヨビ アスパルテーム ノ ドウジ ブンセキホウ

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説明

Simultaneous determination of three artificial sweeteners, neotame (NE), alitame (AL) and aspartame (APM) in various foods by high-performance liquid chromatography (HPLC) was developed. Chopped or homogenized samples were packed into cellulose tubing with 0.01 mol/L hydrochloric acid containing 10% sodium chloride, and dialyzed against 0.01 mol/L hydrochloric acid for 24-48 hours. The dialyzate was passed through an Oasis® MCX cartridge, and the cartridge was washed with water and methanol. Then the three sweeteners were eluted from the cartridge with a mixture of 0.5 mol/L ammonium chloride-acetonitrile (3 : 2). The sweeteners were separated on a Cosmosil 5C18-AR column using a gradient mode with a mobile phase of 0.01 mol/L phosphate buffer (pH 4.0)-acetonitrile and were detected at 210 nm.<br>The recoveries of NE, AL and APM from 8 kinds of foods spiked with 10 and 100 μg/g were 86-100% and 89-104%, respectively. The detection limits of NE, AL and APM were 1 μg/g in samples. Furthermore, the three sweeteners were successfully identified by using liquid chromatography with tandem mass spectrometry.

収録刊行物

  • 食品衛生学雑誌

    食品衛生学雑誌 49 (1), 31-36, 2008

    公益社団法人 日本食品衛生学会

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