高速液体クロマトグラフィーによる油脂中のビタミンAとβ-カロチンの同時定量

DOI Web Site 被引用文献1件

書誌事項

タイトル別名
  • Concurrent Determination of Vitamin A and β-Carotene in Oil and Fat by High Speed Liquid Chromatography
  • コウソク エキタイ クロマトグラフィー ニヨル ユシチュウ ノ ビタミン A

この論文をさがす

抄録

A simple method for determination of vitamin A and β-carotene by highspeed liquid chromatography was established. In this analytical method, the sample to be tested, added with pyrene as an internal standard, was saponified and unsaponifiable matter was extracted once with 80ml of ether. The residue obtained by evaporation of ether was dissolved in a solvent of the same composition as that of the mobile phase for the chromatography and this solution was injected. A wavelength variable detector was used to detect vitamin A at 318nm, and then the wavelength was changed to 453 nm to detect β-carotene. From the chromatogram obtained, quantities of vitamin A and β-carotene were calculated from the ratio of peak heights, using pyrene as the internal standard. In crude oil, the values showed a slight positive error due to interference of substances originating in the unsaponifiable matter of oil. In the recovery test with purified oil, the rate was 96.5-103.4% for vitamin A and 93.5-95.5% for β-carotene. There was no significant difference between individuals carrying out the analysis. This method was applied for the analysis of commercial margarine and butter, and comparison of the values obtained with the known analytical values showed that the values for vitamin A and β-carotene by this method were satisfactory, indicating usefulness of this method of analysis.

収録刊行物

  • 食品衛生学雑誌

    食品衛生学雑誌 18 (6), 487-492_1, 1977

    公益社団法人 日本食品衛生学会

被引用文献 (1)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ