Plate punch method as a simplified detection method for staphylococcal enterotoxins.

DOI
  • ODA Takahiro
    Fukuoka Municipal Institute of Food Sanitation Inspection

Bibliographic Information

Other Title
  • ぶどう球菌エンテロトキシン簡易検査法の応用に関する研究　Ｉ　　平板パンチ法による黄色ぶどう球菌のエンテロトキシン簡易型別法
  • ブドウ球菌エンテロトキシン簡易検出法の応用に関する研究 (第1報)

Abstract

An improved method for rapid determination of enterotoxin-type of Staphylococcus aureus was developed. The procedure includes the following steps: a punched-out piece with a diameter of 8-10mm around a colony of S. aureus on the Baird-Parker medium was put in 0.5ml of phosphate buffer solution containing 0.5% bovine albumin and 2% rabbit serum. Enterotoxin (s) and coagulase extracted into the solution were detected by a reversed passive latex agglutination method using a “SET-RPLA” kit and by a micro-coagulase test, respectively.<BR>The new method showed good agreement with the usual shaking culture method: 95% in terms of enterotoxin producibility and 84% in terms of the type specificity of S. aureus. In the coagulase test, the degrees of agreement with the tube method using brain-heart infusion broth containing 5% rabbit plasma were 95% in terms of coagulase producibility and 100% in terms of coagulase typing.

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Keywords

Details 詳細情報について

  • CRID
    1390001204225083904
  • NII Article ID
    130003692532
  • DOI
    10.3358/shokueishi.26.29
  • ISSN
    18821006
    00156426
  • Text Lang
    ja
  • Data Source
    • JaLC
    • Crossref
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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