Simultaneous Determination of Nine Kinds of Preservatives in Foods by HPLC
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- KISHI Hiroko
- Kanagawa Prefectural Institute of Public Health
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- YAMADA Toshiharu
- Kanagawa Prefectural Institute of Public Health
Bibliographic Information
- Other Title
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- HPLCによる食品中の9種保存料の一斉分析
- HPLC ニ ヨル ショクヒンチュウ ノ 9シュ ホゾンリョウ ノ イッセイ ブンセキ
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Abstract
A simple and rapid HPLC method was developed for the simultaneous determination of nine kinds of preservatives, benzoic acid (BA), sorbic acid (SOA), dehydroacetic acid (DHA), methyl p-hydroxybenzoate (PHBA-Me), ethyl p-hydroxybenzoate (PHBA-Et), isopropyl p-hydroxybenzoate (PHBA-isoPu), propyl p-hydroxybenzoate (PHBA-Pu), isobutyl p-hydroxybenzoate (PHBA-isoBu) and butyl p-hydroxybenzoate (PHBA-Bu), in foods. For solid foods, the preservatives were extracted with methanol. After addition of 5 mmol/L citrate buffer to the extract, the extract solution was cleaned up on an Oasis HLB cartridge. The cartridge was washed with 5 mmol/L citrate buffer and methanol-5 mmol/L citrate buffer (4 : 6). Then, nine kinds of preservatives were eluted with methanol. The eluent was used for BA, SOA and DHA determination by HPLC. Furthermore, a part of the eluent was cleaned up on a Bond Elut PSA cartridge for p-hydroxybenzoate esters determination by HPLC. Liquid foods were cleaned up after addition of 5 mmol/L citrate buffer without the extraction process, and the subsequent procedure was the same as for solid foods. The recoveries of p-hydroxybenzoate esters from ten kinds of foods fortified at levels of 0.01 and 0.10 g/kg each were 91.5 to 107.4%, and those of BA, SOA and DHA were 76.4 to 104.8%. The quantitation limits of the preservatives in foods were 0.005 g/kg.
Journal
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- Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi)
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Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi) 48 (3), 58-63, 2007
Japanese Society for Food Hygiene and Safety
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Details 詳細情報について
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- CRID
- 1390001204225197440
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- NII Article ID
- 10019974295
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- NII Book ID
- AN00117741
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- COI
- 1:CAS:528:DC%2BD2sXotF2hsLo%3D
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- ISSN
- 18821006
- 00156426
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- NDL BIB ID
- 8885396
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- Text Lang
- ja
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- Data Source
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- JaLC
- IRDB
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed