Determination of Polysorbates in Foods by Solid-Phase Extraction and High-Performance Liquid Chromatography

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  • 固相抽出およびＨＰＬＣを用いた食品中のポリソルベートの分析法
  • コソウ チュウシュツ オヨビ HPLC オ モチイタ ショクヒンチュウ ノ ポリソルベート ノ ブンセキホウ

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A simple and rapid method using refractive index high-performance liquid chromatography (RI-HPLC) was developed for the determination of polysorbates (PS) in processed foods. PS were extracted with ethyl acetate containing 5% methanol. The extract was cleaned up on a multimode cartridge (300 mg) and an Alumina-N cartridge (500 mg) to remove fats and food color. HPLC separation was performed on a C18 column (4.6 i.d.×150 mm) with methanol as the mobile phase. The recoveries of PS80 from nine kinds of foods fortified at the levels 1-5 g/kg were 80-99%. The limit of quantitation for PS80 in foods was 0.10 g/kg. The proposed method was applied to Worcestershire sauce that was PS-positive by TLC, and PS was confirmed to be present as PS80 at the concentration of 0.13 g/kg.

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