Discrimination of Psychoactive Fungi (Commonly Called "Magic Mushrooms") Based on the DNA Sequence of the Internal Transcribed Spacer Region.
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- MARUYAMA Takuro
- National Institute of Health Sciences
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- SHIROTA Osamu
- National Institute of Health Sciences
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- KAWAHARA Nobuo
- National Institute of Health Sciences
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- YOKOYAMA Kazumasa
- Faculty of Education, Shiga University
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- MAKINO Yukiko
- Kanto-Shin'etsu Regional Narcotic Control Office, Ministry of Health, Labor and Welfare
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- GODA Yukihiro
- National Institute of Health Sciences
Bibliographic Information
- Other Title
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- 内部転写スペーサー領域の塩基配列解析による幻覚性キノコ(いわゆるマジックマッシュルーム)の基原種の分類
- ナイブ テンシャ スペーサー リョウイキ ノ エンキ ハイレツ カイセキ ニ ヨル ゲンカクセイ キノコ イワユル マジックマッシュルーム ノ キ ゲンシュ ノ ブンルイ
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Abstract
‘Magic mushrooms’ (MMs) are psychoactive fungi containing the hallucinogenic compounds, psilocin (1) and psilocybin (2). Since June 6, 2002, these fungi have been regulated by the Narcotics and Psychotropics Control Law in Japan. Because there are many kinds of MMs and they are sold even as dry powders in local markets, it is very difficult to identify the original species of the MMs by morphological observation. Therefore, we investigated the internal transcribed spacer (ITS) region in the ribosomal RNA gene of MMs obtained in Japanese markets to classify them by a genetic approach. Based on the size and nucleotide sequence of the ITS region amplified by PCR, tested MMs were classified into 6 groups. Furthermore, a comparison of the DNA sequences of the MMs with those of authentic samples or with those found in the databases (GenBank, EMBL and DDBJ) made it possible to identify the species of tested MMs. Analysis by LC revealed that psilocin (1) was contained at the highest level in Panaeolus cyanescens among the MMs, but was absent in the Amanita species.
Journal
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- Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi)
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Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi) 44 (1), 44-48, 2003
Japanese Society for Food Hygiene and Safety
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Keywords
Details 詳細情報について
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- CRID
- 1390001204225413248
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- NII Article ID
- 10011029757
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- NII Book ID
- AN00117741
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- COI
- 1:CAS:528:DC%2BD3sXisFCmt7k%3D
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- ISSN
- 18821006
- 00156426
- http://id.crossref.org/issn/00156426
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- NDL BIB ID
- 6484393
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- PubMed
- 12749196
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- Text Lang
- ja
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- Data Source
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- JaLC
- IRDB
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed