蛍光法による腸炎ビブリオを含む2, 3のビブリオ属細菌の迅速検査法

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タイトル別名
  • A Fluorogenic Assay for the Rapid Detection of Some <i>Vibrio</i> Species Including <i>Vibrio parahaemolyticus</i> in Foods
  • ケイコウホウ ニ ヨル チョウエンビブリオ オ フクム 2 3 ノ ビブリオゾ

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抄録

A rapid assay method for some Vibrio species including Vibrio parahaemolyticus was developed. The assay involved the enrichment culture of Vibrio species in BSP-HP medium (0.2% bacto-tryptone, 2% sodium chloride, 250 units/ml polymyxin B sulfate, 0.025% sodium hexametaphosphate, pH 8.5) and the specific measurement of intracellular trypsin-like activity by using the fluorogenic substrate benzoyl-L-arginine-7-aminomethylcoumarin. In BSP-HP medium, growth and trypsin-like activity of bacteria other than V. parahaemolyticus, V. alginolyticus and V. harveyi were suppressed. Although trypsin-like enzyme in food samples interfered with the fluorogenic assay, the interference could be overcome by the addition of 1mM EDTA to the reaction mixture. Using this fluorogenic assay with commercial seafoods, 400 cells of V. parahaemolyticus per gram of food sample were detected within 8hr. Trypsinlike activity measured by the assay was proportional to the number of V. parahaemolyticus determined by the conventional BTB teepol agar plating method (correlation coefficient r=0.97). In the medium, V. alginolyticus, one of the V. parahaemolyticus-allied bacteria, and V. harveyi also showed vigorous growth and high trypsin-like activity. A more specific culture condition for V. parahaemolyticus is required, and further investigation is in progress.

収録刊行物

  • 食品衛生学雑誌

    食品衛生学雑誌 30 (6), 534-541_1, 1989

    公益社団法人 日本食品衛生学会

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