Analytical Method of Clofencet in Animal Fishery Products by LC/MS

AOYAGI Mitsutoshi, NIIYAMA Kazuhito, NEMOTO Satoru

doi:10.3358/shokueishi.52.156

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ＬＣ／ＭＳによる畜水産物中のクロフェンセットの分析法
LC MS ニヨルチクスイサンブツチュウノクロフェンセットノブンセキホウ

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Abstract

A method for the determination of clofencet in animal and fishery products was developed, using liquid chromatography with electrospray ionization mass spectrometry (LC/ESI-MS). The sample was homogenized with water and hexane, and the homogenate was extracted with acetonitrile, then acetonitrile-water (4 : 1). An aliquot of the crude extract was passed through a C18 cartridge column (1,000 mg), and the eluate was concentrated. A solution of 10% sodium chloride and 1% sodium hydrogen carbonate, and ethyl acetate were added to the residue, and the mixture was shaken. After shaking, the aqueous phase was recovered and acidified with hydrochloric acid, and then clofencet was extracted with ethyl acetate. The extract was evaporated to dryness and the residue was dissolved in methanol-water (3 : 7). Clofencet was analyzed by LC/MS. The recoveries of clofencet from ten kinds of animal and fishery products were 77.8-97.8%, and the relative standard deviations were 0.6-5.8% (n=5). S/N of the peak of clofencet was >10 and no interfering peak was found in animal and fishery products fortified at 0.01 mg/kg.

Journal

Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi)

Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi) 52 (3), 156-160, 2011

Japanese Society for Food Hygiene and Safety

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