Development of the Mouse Passive Abdominal Wall Anaphylaxis (PAA) Method and Application of the Method to Search for Anti-allergic Effect of Foods

FUKUI Hisae, KATAOKA Hiromi, SEMMA Masanori, ITO Yoshio, TAKAGAKI Yutaka

doi:10.3358/shokueishi.39.4_251

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マウスを用いた受身腹壁アナフィラキシー (PAA) 反応の開発と食品の抗アレルギー効果検索への応用
マウスオモチイタウケミフクヘキアナフィラキシー PAA ハンノウノ

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Abstract

Food allergy, which generally belongs to the category of Type I hypersensitivity reaction, is a serious health problem. Previously, we reported two types of mouse anaphylactic models, one using the abdominal wall as a site for both induction and estimation (AW method), and the other estimating the hypotension in mice sensitized passively with mouse monoclonal anti-DNP IgE.<br>In this paper, we propose a passive abdominal wall anaphylaxis (PAA) method for use in screening for anti-allergic substances. Normal mice were intraperitoneally sensitized with mouse monoclonal anti-DNP IgE (5μg/mouse) and 24 hours later, challenged with dinitrophenyl human serum albumin (10μg/50μL/site) on the abdominal wall. The PAA was estimated in terms of the increase of vascular permeability at the challenged abdominal wall site.<br>The PAA was inhibited by the oral preadministration of diphenhydramine in a dosedependent manner (PAA inhibition, PAAI). The anti-allergic activities of such well-known anti-allergic foods as oolong tea, garlic, and leek were confirmed by the PAAI test. Therefore, the PAA method appears to be applicable for the screening of anti-allergic substances in foods, simply, rapidly, and with high sensitivity.

Journal

Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi)

Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi) 39 (4), 251-255_1, 1998

Japanese Society for Food Hygiene and Safety

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