Simplified Classification Method for Residual Antibiotics by Microbiological Assay Using Drug-Resistant Bacteria

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  • YOSHIDA Kenichi
    Department of Veterinary Public Health, Faculty of Agriculture, Miyazaki University
  • KONDO Fusao
    Department of Veterinary Public Health, Faculty of Agriculture, Miyazaki University

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  • 薬剤耐性菌を用いる残留抗生物質の微生物学的簡易分別同定法
  • ヤクザイ タイセイキン オ モチイル ザンリュウ コウセイ ブッシツ ノ ビセ

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Abstract

We prepared oxytetracycline (OTC)-resistant Bacillus cereus (50μg/ml), tylosin (TS)-resistant B. subtilis (12.5μg/ml) and streptomycin (SM)-resistant B. subtilis (100μg/ml) and used the sensitive organisms for microbiological assay to detect residual antibiotics. The development of drug-resistant strains to OTC, TS and SM was carried out by the broth dilution method and the obtained drug-resistant bacteria were investigated for cross resistance to about 19 antibiotics. Each drug-resistant bacterium showed almost the same cross resistance within the same groups of antibiotics.<br>The results suggested that the application of these three drug-resistant strains and the sensitive organisms will make it possible to classify residues into tetracyclines, macrolides and aminoglycoside antibiotics, especially streptomycin.

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