Investigation of Whether CLSI Broth Microdilution Method is Applicable for MICs Determination of <i>Enterococcus</i> species

HASHIMOTO Takeshi, HASHIMOTO Sayaka, MATSUZAKI Manabu, SEKIGUCHI Yoshihiro, HASHIMOTO Yoshiyasu, ASAO Miyuki, TAKAGI Masami

doi:10.3358/shokueishi.53.225

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腸球菌の微量液体希釈法を用いた薬剤感受性試験の検討
チョウキュウキンノビリョウエキタイキシャクホウオモチイタヤクザイカンジュセイシケンノケントウ

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Abstract

The broth microdilution (BMD) method is an antimicrobial susceptibility testing method defined as a guideline by the Clinical and Laboratory Standards Institute (CLSI). To date, the Japanese Veterinary Antimicrobial Resistance Monitoring System (JVARM), has adopted the agar dilution (AD) method for monitoring antimicrobial resistances targeting isolates of Enterococcus spp., found in the fecal flora of food-producing animals, as indicator bacteria. However, the AD method is tedious, and time-consuming. In order to examine whether it could be replaced with the BMD method, the two methods were compared in terms of the correlation of MICs. In this study, the BMD results agreed with the AD results within ±1 log₂ dilutions in 72.3% of cases, except for the antimicrobial feed additive, Nosiheptide (NHT). Similarly, except for NHT, the MIC₅₀ and MIC₉₀ values obtained by the two methods were well correlated. In conclusion, our results indicate that the BMD method might be suitable for antimicrobial susceptibility testing targeting Enterococcus spp..

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Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi)

Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi) 53 (5), 225-232, 2012

Japanese Society for Food Hygiene and Safety

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