Rapid Multi-Residue Analysis of Pesticides in Pulses by LC-MS/MS
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- IWAKOSHI Keiko
- Tokyo Metropolitan Institute of Public Health
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- OTSUKA Kenji
- Tokyo Metropolitan Institute of Public Health
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- TAMURA Yasuhiro
- Tokyo Metropolitan Institute of Public Health
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- TOMIZAWA Sanae
- Tokyo Metropolitan Institute of Public Health
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- MASUBUCHI Tamako
- Tokyo Metropolitan Institute of Public Health
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- YAMAKI Yumiko
- Tokyo Metropolitan Institute of Public Health
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- NAKAGAWA Yukiko
- Tokyo Metropolitan Institute of Public Health
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- MASUDA Ryoko
- Tokyo Metropolitan Institute of Public Health
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- SUTO Shota
- Tokyo Metropolitan Institute of Public Health
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- KOKAJI Yoshie
- Tokyo Metropolitan Institute of Public Health
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- SHINDO Tetsuya
- Tokyo Metropolitan Institute of Public Health
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- TAKANO Ichiro
- Tokyo Metropolitan Institute of Public Health
Bibliographic Information
- Other Title
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- LC-MS/MSを用いた豆類中残留農薬の迅速分析法に関する研究
- LC-MS/MS オ モチイタ マメルイ チュウ ザンリュウ ノウヤク ノ ジンソク ブンセキホウ ニ カンスル ケンキュウ
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Abstract
<p>Rapid multi-residue analysis of pesticides in pulses was developed using LC-MS/MS. Pesticide residues in 5 g of homogenized pulses were extracted with 30 mL of acetonitrile and salted out with 4 g of anhydrous magnesium sulfate and 2 g of sodium chloride in the presence of citrate buffer in a disposable tube. The resulting residues were extracted with 30 mL of acetonitrile, and co-extractives were removed on a handmade four-layer column, consisting of a layer of Z-Sep/C18 (20 mg/50 mg) dry particles on top of a three-layer, custom-made (pre-packed) column (lower bed: 60 mg of PSA, middle bed: 30 mg of GC, and top bed: 60 mg of C18) packed in a 10 mm internal diameter polypropylene column (3 mL). The developed method showed good recoveries of pesticides in soybean, lentil, white kidney bean and garbanzo. According to the method validation guideline of the Ministry of Health, Labour and Welfare of Japan, recovery tests were conducted in soybeans fortified with 107 kinds of pesticides at the levels of 0.01 and 0.1 μg/g, respectively. At each concentration 2 samples were extracted on 5 separate days. Pesticides in the test solution were determined by LC-MS/MS using scheduled MRM. As regards the trueness of this method for 107 pesticides in soybeans, 97 pesticides were in the range of 70–120% with satisfactory repeatability and within-run reproducibility. This new method is expected to be applicable for routine examination of pesticide residues in soybeans.</p>
Journal
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- Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi)
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Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi) 57 (5), 150-154, 2016
Japanese Society for Food Hygiene and Safety
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Details 詳細情報について
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- CRID
- 1390001204228376832
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- NII Article ID
- 130005249719
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- NII Book ID
- AN00117741
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- ISSN
- 18821006
- 00156426
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- NDL BIB ID
- 027725746
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- PubMed
- 27784865
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- Text Lang
- ja
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- Data Source
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- JaLC
- IRDB
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed