Measurement of isoelectric points of phospholipid exchange proteins by gel isoelectric focusing.

DOI Web Site Web Site PubMed
  • YAMADA KEIKO
    The Second Division, Department of Biochemistry, Sapporo Medical College
  • SASAKI TERUKATSU
    The Second Division, Department of Biochemistry, Sapporo Medical College
  • SAKAGAMI TOSHIO
    The Second Division, Department of Biochemistry, Sapporo Medical College

Bibliographic Information

Other Title
  • Measurement of Isoelectric Points of Ph

Search this article

Abstract

YAMADA, K, SASAKI, T. and SAKAGAMI. T, Measurement of Isoelectric Points of Phospholipid Exchange Proteins by Gel Isoelectric Focusing. Tohoku J. exp. Med., 1981, 135 (1), 37-42 - A method of the estimation of isoelectric points of phospholipid exchange proteins is described. The phospholipid presumably bound to a phospholipid exchange protein was replaced with [3H]phosphatidylcholine of a high specific radioactivity by an incubation of the protein with liposomes containing the labeled lipid and dimannosyl diglyceride. After the incubation, a major portion of the liposomes was separated from the protein by an affinity of the liposomes to concanavalin A-Sepharose 2B. The isoelectric point of the protein was measured by gel isoelectric focusing of the protein, which was located by tritium radioactivity of the bound [3H]phosphatidylcholine. The method was used to measure isoelectric points of partially purified phospholipid exchange proteins in pig liver.

Journal

Keywords

Details 詳細情報について

Report a problem

Back to top