Extraction of hyaluronic acid from rabbit skin with lanthanum chloride.
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- MUNAKATA HIROSHI
- Department of Biochemistry, Tohoku University School of Medicine
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- YOSIZAWA ZENSAKU
- Department of Biochemistry, Tohoku University School of Medicine
書誌事項
- タイトル別名
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- Extraction of Hyaluronic Acid from Rabb
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抄録
The defatted rabbit skin was extracted with 0.5M LaCl3, and the extract was dialyzed exhaustively against distilled water. The precipitate formed during dialysis was dissolved in 28mM EDTA (ethylenediamine tetraacetate) (pH 7.0). Fr. A was obtained from the solution by precipitation with ethanol in the presence of sodium acetate. The results of gel filtration on Sepharose 4B, electrophoresis on cellulose acetate membrane before and after digestion with Streptomyces hyaluronidase, and analytical data indicated that Fr. A was hyaluronic acid with high molecular weight. The present observation together with previous findings suggest that the binding status of proteoglycans in the skin differs significantly from that in cartilages.
収録刊行物
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- The Tohoku Journal of Experimental Medicine
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The Tohoku Journal of Experimental Medicine 132 (3), 337-340, 1980
東北ジャーナル刊行会
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詳細情報 詳細情報について
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- CRID
- 1390001204240777088
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- NII論文ID
- 130003407731
- 40005306686
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- NII書誌ID
- AA00863920
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- COI
- 1:CAS:528:DyaL3MXmtFWitQ%3D%3D
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- ISSN
- 13493329
- 00408727
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- NDL書誌ID
- 2337503
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- PubMed
- 7466813
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可