Establishment and Cytological Observation of a Cell Line from Rat Fibrosarcoma Induced by 3-Methylcholanthrene

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Abstract

A cell line from fibrosarcoma induced in rat by 3-methylchoranthrene was established under stationary culture conditions. The cell line (MC-1 cell line) was maintained with 70 subcultures in vitro for more than two years. The monolayer of the cell line was composed of fibroblastic cells. Electron microscopic observations revealed abundant cytoplasmic filaments and extracellular collagen fibers in the slowly growing state, but scarce fibrils in the rapidly growing state. The cells actively phagocytized extracellular proteins and particles. The amount of mucopolysaccharides on the cell membane increased significantly as compared with other cell lines investigated by ruthenium red stain method and sialic acid assay, and considerably increased in inverse proportion to diminution of growth in the MC-1 cell line.<br> These morphologic and functional features of the MC-1 cell line altered under some cultural conditions in proportion to its growth rate, and few truly specific signs of the establishment of a cell line except endless cell multiplication after transfers have been revealed by the present experiments.

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