Application of Precipitating Activity of <i>Canavalia lineata</i> DC Lectin for Isolation of α<sub>2</sub>-Macroglobulin from Human Plasma
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- IWASA MINEO
- Department of Legal Medicine, Gifu University School of Medicine
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- SAGISAKA KAORU
- Department of Legal Medicine, Gifu University School of Medicine
Bibliographic Information
- Other Title
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- Application of Precipitating Activity of Canavalia lineata DC Lectin for Isolation of α2-Macroglobulin from Human Plasma
- Application of Precipitating Activity o
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Description
To isolate α2-macroglobulin from human plasma, complex of Canavalia lineata DC lectin and plasma was used as a starting material. The complex was solubilized with 0.08 M phosphate buffer pH 7.2 containing 0.9% NaCl and 0.02 M α-methyl-D-mannoside. The solution was applied onto a Bio gel A-5m column and eluted with solubilizing buffer. Of three peaks, only the second peak contained α2macroglobulin. After equilibrating with 0.06 M potassium phosphate buffer pH 6.8, the fraction was applied onto a hydroxylapatite column and eluted with the same buffer. The eluate was confirmed to be extremely pure α2-macroglobulin; immunoelectrophoresis with anti-human serum produced a single precipitation line, and SDS disc electropho-resis gave a single band at α-globulin region. Few differences in carbohydrate composition between this preparation and those prepared by other methods were observed. By this method, about 45% of armacroglobulin present in plasma was recovered.
Journal
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- The Tohoku Journal of Experimental Medicine
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The Tohoku Journal of Experimental Medicine 130 (2), 165-173, 1980
Tohoku University Medical Press
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Details 詳細情報について
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- CRID
- 1390001204241743744
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- NII Article ID
- 130003492682
- 40005306577
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- NII Book ID
- AA00863920
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- COI
- 1:CAS:528:DyaL3cXkt1Cjurc%3D
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- ISSN
- 13493329
- 00408727
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- NDL BIB ID
- 2208944
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- PubMed
- 6155714
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed