Comparative Studies of Non-Hemoglobin Proteins of Adult and Newborn Red Blood Cell Lysates

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  • SAGISAKA KAORU
    Department of Legal Medicine, Gifu University School of Medicine
  • IWASA MINEO
    Department of Legal Medicine, Gifu University School of Medicine
  • YAMASHITA HIROKO
    Department of Legal Medicine, Gifu University School of Medicine
  • YAMADA SADAO
    Department of Legal Medicine, Gifu University School of Medicine

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タイトル別名
  • Comparative Studies of Non Hemoglobin P

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It has been known that hemolysate is composed of not only hemoglobin but also non-hemoglobin proteins (NHP). NHP isolated effectively from hemolysate of adult and newborn by CM-Sephadex chromatography was investigated by polyacrylamide disc or slab electrophoresis. It was demonstrated on slab electrophoresis that adult NHP is composed of 4 zones (approximately 14 bands) with different mobilities and that adult NHP shows a considerably different pattern from cord NHP. The difference was observed also on the electrophoresis stained for esterase or lactate dehydrognease. Rabbit antiserum to adult NHP was adsorbed with cord NHP and specific antiserum for adult NHP was prepared. However, antiserum reacting with only cord NHP was not obtained from anti-cord NHP serum. These results suggested that adult specific components with passable antigenicity were present in adult NHP and that cord NHP had similar antigenicity as adult NHP.

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