Assay of serum E2 concentration in postmenopausal breast cancer patients using a high-sensitivity RIA method is generally useful

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  • Morimoto Masami
    Department of Oncological and Regenerative Surgery, Institute of Health Biosciences, the University of Tokushima
  • Takahashi Masako
    Department of Surgery, Tokushima Breast Care Clinic
  • Honda Junko
    Department of Surgery, Higashitokushima National Hospital
  • Yoshida Takahiro
    Department of Oncological and Regenerative Surgery, Institute of Health Biosciences, the University of Tokushima
  • Yoshida Mitsuteru
    Department of Oncological and Regenerative Surgery, Institute of Health Biosciences, the University of Tokushima
  • Toba Hiroaki
    Department of Oncological and Regenerative Surgery, Institute of Health Biosciences, the University of Tokushima
  • Imoto Issei
    Department of Human Genetics, Institute of Health Biosciences, the University of Tokushima Graduate School
  • Tangoku Akira
    Department of Oncological and Regenerative Surgery, Institute of Health Biosciences, the University of Tokushima
  • Sasa Mitsunori
    Department of Surgery, Tokushima Breast Care Clinic

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<p>Background: Serum E2 must be monitored for aromatase inhibitor (AI) therapy, but conventional assays lack sensitivity. Subjects/Methods: Forty amenorrheic breast cancer patients scheduled for AI treatment but requiring hormonological confirmation of their menopausal status were studied. Serum E2 data generated by high-sensitivity RIA and by LC-MS/MS were analyzed for correlation. Results: RIA gave a higher E2 value than LC-MS/MS in 62% of cases, but there was a significant positive correlation. Patients whose E2 levels by RIA were ≥ 2.5 pg/mL higher than those by LC-MS/MS (RIA-H group) and all other patients (RIA-N group) were compared. Both groups showed strong correlations between the two assay methods. With both methods patients with a high BMI had significantly elevated E2. Multiple regression analysis used age, age at menarche, number of births and BMI as explanatory variables. Significant variables were the BMI with LC-MS/MS, and both BMI and age with RIA. The RIA-H and RIA-N groups showed no difference in regard to the BMI, whereas the age was significantly lower in the RIA-H group. Summary: Serum E2 levels determined for postmenopausal women by RIA and LC-MS/MS generally correlated well. High-sensitivity RIA is a potentially useful clinical assay, but it overestimated serum E2 in some women. J. Med. Invest. 63: 236-240, August, 2016</p>

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