Integration of Flow Injection Analysis and Zeptomole-Level Detection of the Fe(II)-o-Phenanthroline Complex

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  • SATO Kiyoshi
    Department of Applied Chemistry, Graduate School of Engineering, The University of Tokyo
  • TOKESHI Manabu
    Integrated Chemistry Project, Kanagawa Academy of Science and Technology
  • KITAMORI Takehiko
    Department of Applied Chemistry, Graduate School of Engineering, The University of Tokyo Integrated Chemistry Project, Kanagawa Academy of Science and Technology
  • SAWADA Tsuguo
    Department of Applied Chemistry, Graduate School of Engineering, The University of Tokyo

書誌事項

タイトル別名
  • Integration of Flow Injection Analysis and Zeptomole-Level Detection of the Fe(II)-<i>o</i>-Phenanthroline Complex
  • Integration of Flow Injection Analysis and Zeptomole-Level Detection of the Fe 2 o Phenanthroline Complex

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抄録

Microchannels having a 150×100 µm cross section were fabricated in a quartz glass chip as a component in an integrated flow injection analysis (FIA) system. They were put to use for flow, mixing, reaction, and detection. The reaction system was a chelating reaction of divalent iron ion with o-phenanthroline (o-phen), and a photothermal microscope was applied for the ultra-sensitive detection of the non-fluorescent reaction product. Nano liter levels of solutions were introduced and transported by capillary action and mixed by molecular diffusion. Zeptomole levels of the reaction product were detected quantitatively. This was the first demonstration of an on-chip chemical determination device which integrates the primitive FIA system without using electroosmotic liquid control or fluorometric determination.

収録刊行物

  • Analytical Sciences

    Analytical Sciences 15 (7), 641-645, 1999

    社団法人 日本分析化学会

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