Suppressive mechanisms of bacterial wilt in tomato plants grown in repeatedly used pumice

Matsuoka Tomoo, Toyota Koki, Sato Kazuo, Masuda Kazunari, Kuroda Tetsuo

doi:10.18946/jssm.59.1_15

We identified the microorganisms involved in the suppression of bacterial wilt in tomato plants grown in pumice which had been used for 13 years for continuous hydroponic culture. The results of the substrate-induced respiration inhibition method indicated that bacteria may be the predominant microorganism communities in the used pumice. Fifty bacterial colonies were isolated from tomato roots grown in the used pumice and their inhibition of the growth of Ralstonia solanacearum was tested in tomato root exudates as first screening. Five isolates selected in the first screening were inoculated to new pumice infested with the pathogen as second screening. Only one isolate, designated as Burkholderia sp. W3, effectively suppressed the disease. While the suppressive effect of the used pumice was lost by autoclaving, it recovered by the inoculation of the W3 strain. The incidence of bacterial wilt in the new pumice also decreased by the inoculation of the W3 strain, and the suppressiveness of the W3 strain was enhanced by the addition of glucose. Bacterial community structure of tomato roots was compared between the used and new pumice by PCR-DGGE analysis. The banding patterns were different between the two types of pumices. The band corresponding to the W3 strain was observed in the used pumice, but not in the new pumice. These results suggest that the W3 strain is involved in the suppression of bacterial wilt of tomato by the used pumice.

Suppressive mechanisms of bacterial wilt in tomato plants grown in repeatedly used pumice

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