Transduction of Analytical Signals by Supramolecular Assemblies of Amphiphiles Containing Heterogeneously Distributed Fluorophores.
-
- BRENNAN John D.
- Department of Chemistry, Brock University
-
- BROWN R. Stephen
- Department of Chemistry, Queen’s University
-
- KRULL Ulrich J.
- Chemical Sensors Group, Department of Chemistry, Erindale Campus, University of Toronto
書誌事項
- タイトル別名
-
- Transduction of Analytical Signals by S
この論文をさがす
抄録
We report on the development and characterization of supramolecular assemblies of amphiphiles which are able to transduce changes of pH into optical signals. A series of assemblies have been investigated, including vesicular bilayer lipid membranes, monolayer films of fatty acids or phospholipids at the air-water interface, and covalently immobilized alkylsilane monolayers. In all cases, these membrane assemblies were formed from mixtures of species having zwitterionic and ionizable headgroups. In addition, a small amount of the fluorescently labeled phospholipid nitrobenzoxadiazole phosphatidylethanolamine (1 - 3%) was incorporated into the membranes. This resulted in systems which were able to transduce alterations of pH into changes in fluorescence intensity. Investigations into the physical mechanism responsible for alterations of fluorescence intensity from such membranes suggested that the nitro-benzoxadiazole phosphatidylethanolamine (NBD-PE) signal was based on changes of self-quenching of the probe which occurred when the physical and electrostatic characteristics of the membranes were altered. Useful signals were obtained only when microscopic phase separation existed within the membranes, indicating that careful design of the membrane was required to optimize the signal generated.
収録刊行物
-
- Analytical Sciences
-
Analytical Sciences 14 (1), 141-149, 1998
社団法人 日本分析化学会
- Tweet
キーワード
詳細情報 詳細情報について
-
- CRID
- 1390001204253461632
-
- NII論文ID
- 10002414436
-
- NII書誌ID
- AA10500785
-
- COI
- 1:CAS:528:DyaK1cXhtV2qtb0%3D
-
- ISSN
- 13482246
- 09106340
-
- NDL書誌ID
- 4406436
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可